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大满贯667171com:教师简介

赵晓璐
时间:2016-12-26 12:33:10 阅读量:
  

职称职务:副教授、硕士生导师、351人才计划珞珈青年学者
学科专业:蛋白质组学/代谢组学
研究方向:胆固醇代谢和蛋白质组学/代谢组学
实验室位置:大满贯667171com6110室
联系电话:027-68753797
Email: zhaoxiaolu@whu.edu.cn
 

学习经历
2004/08-2011/05:University of Southern Denmark, 生物化学与分子生物学,硕士(M.S.)&博士(Ph.D.)
1999/09-2003/07:大满贯667171com,药学,学士(B.S.

主要工作经历及任职情况
2016/2-2016/8,美国宾夕法尼亚大学,生物化学和生物物理学系,访问副教授
2014/11-至今 大满贯667171com,大满贯667171com,副教授
2012/02-2014/10,大满贯667171com,大满贯667171com,讲师


主要社会兼职
第十一届中国蛋白质组学大会组织委员会委员


主要研究领域及兴趣


运用蛋白质组学/代谢组学手段研究蛋白及修饰与代谢物小分子在胆固醇代谢调控和疾病中的关键作用,建立了针对不同蛋白及多种翻译后修饰(磷酸化、乙酰化、甲基化、泛素化、胆固醇修饰等)和不同性质的代谢物分子(亲水、亲脂)的高灵敏高通量分析方法,并应用到不同代谢疾病模型的研究中。

技术专长
1)蛋白质组学/Proteomics

已建立多种蛋白翻译后修饰的分析方法,多种蛋白定量技术和互作蛋白质组学等分析手段。

2)代谢组学/Metabolomics

已建立基于质谱技术的代谢组学方法,比如基于GC-MS的同位素示踪代谢流分析和基于LC-MS的靶向和非靶向代谢物小分子分析手段


承担课程(本科生、研究生)
主讲:本科生课程《生物化学实验》和《蛋白质组学》;

   研究生课程《蛋白质组学》


主持课题项目
1. 科技部重点研发计划:家族性高胆固醇血症发病新机制及其导致重要器官发育缺陷的研究,2019-2023,课题骨干

2. 国家自然科学基金面上项目:肠道病毒71型介导的宿主组蛋白H3切割及其在感染中的作用研究,2019-2022,主持

3. 湖北省自然科学基金面上项目:组蛋白H3切割在肠道病毒71型感染中的关键作用研究,2018-2019,主持

4. 大满贯667171com交叉学科基金:新型DNA碱基的发现及功能研究,2018-2019

5. 大满贯667171com“351人才计划珞珈青年学者”:基于定量蛋白质组学的组蛋白修饰研究大满贯667171com,2016-2018,主持

6. 国家自然科学基金:组蛋白修饰在视网膜损伤中的关键作用研究,,国家自然科学基金委,2015-2017,主持

7. 教育部留学回国人员科研启动基金:二型糖尿病小鼠线粒体蛋白磷酸化和乙酰化研究,2015-2017,主持

8. 国家自然科学基金:组蛋白修饰在视网膜损伤中的关键作用研究。2015.1-2017.12,主持;

9. 教育部新教师基金:受登革病毒感染调控的宿主蛋白及其翻译后修饰研究,2013-2015,主持;

10. 湖北省自然科学基金:二型糖尿病小鼠线粒体蛋白异常磷酸化研究,2013-2015,主持;


代表性论文:
1) Hu A#, Zhang JZ#, Wang J, Li CC, Yuan M, Deng G, Lin ZC, Qiu ZP, Liu HY, Wang XW, Wei PC, He X, Zhao X*, Qiu WW*, Song BL*. Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate. Cell Res, doi: 10.1038/s41422-022-00622-0, 2022

2)
Miao M*, Yuan M, Xiong X, Qiu Y, Deng G, Yu F, Huang W, Bai S, Zhou X*, Zhao X*. Enterovirus 71 3C proteolytically processes the histone H3 N-terminal tail during infection. Virol Sin, doi:10.1016/j.virs.2022.02.006, 2022

3)
Wang JK, Li Y, Zhao X, Liu YB, Tan J, Xing YY, Adi D, Wang YT, Fu ZY, Ma YT, Liu SM, Liu Y, Wang Y, Shi XJ, Lu XY, Song BL, Luo J. Ablation of Plasma Prekallikrein Decreases LDL Cholesterol by Stabilizing LDL Receptor and Protects against Atherosclerosis. Circulation, doi: 10.1161/CIRCULATIONAHA.121.056491, 2022

4)
Lu XY, Shi XJ, Hu A, Wang JQ, Ding Y, Jiang W, Sun M, Zhao X, Luo J, Qi W, Song BL. Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis. Nature. 588(7838):479-484, 2020.

5)
Li QL, Wang DY, Ju LG, Yao J, Gao C, Lei PJ, Li LY, Zhao X*, Wu M*. The hyper-activation of transcriptional enhancers in breast cancer. Clin Epigenetics. 11(1):48, 2019

6)
Yang F, Wang D, Tong Y, Qin C, Yang L, Yu F, Huang X, Liu S, Cao Z, Guo L, Li W, Wu Y*, Zhao X*.  Thermostable potassium channel-inhibiting neurotoxins in processed scorpion medicinal material revealed by proteomic analysis: Implications of its pharmaceutical basis in traditional Chinese medicine. J Proteomics. doi: 10.1016/j.jprot.2019.103435,2019

7)
Miao M, Yu F, Wang D, Tong Y, Yang L, Xu J, Qiu Y, Zhou X*, Zhao X*. Proteomics Profiling of Host Cell Response via Protein Expression and Phosphorylation upon Dengue Virus Infection. Virol Sin. doi: 10.1007/s12250-019-00131-2, 2019

8)
Li W, Wang HY, Zhao X, Duan H, Cheng B, Liu Y, Zhao M, Shu W, Mei Y, Wen Z, Tang M, Guo L, Li G, Chen Q, Liu X, Du HN*. A methylation-phosphorylation switch determines Plk1 kinase activity and function in DNA damage repair. Sci Adv. 5(3):eaau7566, 2019

9)
Cao L, Ji Y, Zeng L, Liu Q, Zhang Z, Guo S, Guo X, Tong Y, Zhao X, Li CM, Chen Y*, Guo D*. P200 family protein IFI204 negatively regulates type I interferon responses by targeting IRF7 in nucleus. PLoS Pathog. (10):e1008079, 2019

10) You XJ, Liu T, Ma CJ, Qi CB, Tong Y, Zhao X, Yuan BF*, Feng YQ. Determination of RNA Hydroxylmethylation in Mammals by Mass Spectrometry Analysis. Anal Chem. 20;91(16):10477-10483, 2019

11)
Lund PJ, Kori Y, Zhao X, Sidoli S, Yuan ZF, Garcia BA. “Isotopic Labeling and Quantitative Proteomics of Acetylation on Histones and Beyond”. Methods Mol Biol. 1977:43-70, 2019

12)
Guo Q, Sidoli S, Garcia BA*, Zhao X*. Assessment of Quantification Precision of Histone Post-Translational Modifications by Using an Ion Trap and down To 50?000 Cells as Starting Material. J Proteome Res. 17(1):234-242,2018

13)
Shang J, Xia T, Han QQ, Zhao X, Hu MM, Shu HB, Guo L*. Quantitative Proteomics Identified TTC4 as a TBK1 Interactor and a Positive Regulator of SeV-induced Innate Immunity. Proteomics. 18(2). doi: 10.1002/pmic.201700403, 2018

14)
Kori Y, Sidoli S, Yuan Z, Lund P, Zhao X*, Garcia B*. Proteome-wide acetylation dynamics in human cells. Sci. Rep. 7,10296; DOI:10.1038/s41598-017-09918-3, 2017

15)
Wang W, Sidoli S, Zhang W, Wang Q, Wang L, Jensen ON, Guo L, Zhao X*, Zheng L*. Abnormal levels of histone methylation in the retinas of diabetic rats are reversed by minocycline treatment. Sci. Rep. 7, 45103; doi: 10.1038/srep45103, 2017

16)
Li F, Zheng LD, Chen X, Zhao X, Briggs SD, Du HN*. Gcn5-mediated Rph1 acetylation regulates its autophagic degradation under DNA damage stress. Nucleic Acids Res. 45(9):5183-5197, 2017

17)
Zhu X, Li H, Guo L, Zhao X*. Phosphoproteomic Analysis Based on Liquid Chromatography-Tandem Mass Spectrometry. Journal of Analytical Science. 32(1):1066-6144, 2016

18) Li S, Zhu M, Pan R, Fang T, Cao YY, Chen S, Zhao X, Lei CQ, Guo L, Chen Y, Li CM, Jokitalo E, Yin Y, Shu HB, Guo D. The tumor suppressor PTEN has a critical role in antiviral innate immunity.
Nat Immunol. 17(3):241-9, 2016

19)
Xia H, Wang P, Wang GC, Yang J, Sun X, Wu W, Qiu Y, Shu T, Zhao X, Yin L, Qin CF, Hu Y, Zhou X. Human Enterovirus Nonstructural Protein 2CATPase Functions as Both an RNA Helicase and ATP-independent RNA Chaperone. PLoS Pathog. 11(7):e1005067, 2015

20)
Zhao X, Sidoli S, Wang L, Guo L*, Jensen ON*, Zheng L*. Quantitative proteomic analysis of histone PTMs upon ischemia/reperfusion-induced retinal injury. J Proteome Res. 4;13(4): 2175-86, 2014

21)
Zhao X, Bak S, Pedersen AJT, Jensen ON, H?jlund K*. Insulin increases phosphorylation of mitochondrial proteins in human skeletal muscle in vivo. J Proteome Res. 4;13(4): 2359?2369, 2014

22)
Zhang X, Zuo X, Yang B, Li Z, Xue Y, Yu Z, Huang J, Zhao X, Zhou J, Yan Y, Zhang H, Guo P, Guo L, Zhang Y, and Fu X. MicroRNA Enhances Translation in the Mitochondria during Muscle Differentiation. Cell. 158(3):607-19, 2014

23)
Zeng H, Rao X, Zhang L, Zhao X, Zhang W, Wang J, Xu F, Guo L. Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome. J Proteomics. 109C:125-142, 2014

24)
Zhang X, Clausen MR, Zhao X, Zheng H, Bertram HC*. Enhancing the power of liquid chromatography-mass spectrometry-based urine metabolomics in negative ion mode by optimization of the additive. Anal Chem. 18;84(18):7785-92, 2012

25)
Zhao X, Leon IR, Bak S, Mogensen M, Wrzesinski K, Hojlund K*, Jensen ON*. Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes. Mol Cell Proteomics. 10(1):M110.000299, 2011

26) Ye J, Zhang X, Young C, Zhao X, Hao Q, Cheng L, Jensen ON*. Optimized IMAC-IMAC protocol for phosphopeptide recovery from complex biological samples.
J Proteome Res. 2;9(7):3561-73, 2010

             

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